Lentivirus Gene Engineering Protocols

Lentivirus-mediated Gene Expression

ReverX: Reverse Engineering of Protocols

Communication protocols determine how network components interact with each other. Therefore, the ability to derive a speci cation of a protocol can be useful in various contexts, such as to support deeper black-box testing or e ective defense mechanisms. Unfortunately, it is often hard to obtain the speci cation because systems implement closed (i.e., undocumented) protocols, or because a time...

Adherens Junction Formation Inhibits Lentivirus Entry and Gene Transfer

Although cellular signaling pathways that affect lentivirus infection have been investigated, the role of cell-cell interactions in lentiviral gene delivery remains elusive. In the course of our studies we observed that lentiviral gene transfer was a strong function of the position of epithelial cells within colonies. While peripheral cells were transduced efficiently, cells in the center of co...

Lentivirus as a tool for lineage-specific gene manipulations.

The trophectoderm (TE) of blastocysts, the first epithelium established in mammalian development, (1) plays signaling, supportive, and patterning functions during preimplantation development, (2) ensures embryo implantation into the uterine wall, and (3) gives rise to extraembryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive t...

Engineering change protocols for behavioral synthesis

Rapid prototyping and development of in-circuit and FPGA-based emulators as key accelerators for fast time-to-market has resulted in a need for fast error correction mechanisms. The fabricated or emulated prototypes upon error diagnosis require quick and as much as possible flexible engineering change (EC). However, this problem has recently initiated research activity mainly in the logic synth...